DNA “hopping” in a nanoslit post array
Fan-Tso Chien1*, Po-Keng Lin2,1, Cheng-Hsiang Hung2, Ming-Hung Yu1, Yeng-Long Chen1,2,3
1Institute of Physics, Academia Sinica, Taipei, Taiwan
2Department of Physics, National Taiwan University, Taipei, Taiwan
3Department of Chemical Engineering, National Tsing-Hua University, Hsinchu, Taiwan
* presenting author:FAN-TSO CHIEN, email:fantso@gmail.com
Abnormal hopping-mediated DNA trajectories are reported in nano-slit post arrays, in the absence of external forces. Normal Brownian diffusion is found in nanoslits with height h=270nm, and DNA diffusivity decreases as post density increases. The apparent “hopping” is found in nano-slits with h=65nm, in which DNA molecules are observed to absorb to posts. DNA diffusion is coupled with post-adsorption, leading to frequent hopping as the post density increases. The Hidden Markov model is applied to find the most likely hopping probability, allowing characterization of the energy landscape.


Keywords: DNA conformation, polymer confinement, nanofludics