Relaxation of Vascular Endothelial Growth Factor Antibody Coated Magnetic Nanoparticles in Low Fields Nuclear Magnetic Resonance
Yao-Wei Yeh1*, Shu-Hsien Liao2, Chiu-Hsien Wu1, Kuen-Lin Chen1
1Department of Physics, National Chung Hsing University, Taichung, Taiwan
2Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei, Taiwan
* presenting author:Yao-Wei Yeh, email:wei791002@yahoo.com.tw
Magnetic nanoparticles coated with antibody have recently been applied for screening on surgical treatment of tumors1, hyperthermia2, drug delivery3, and magnetic resonance imaging (MRI)4. The common indicators in the cancer detection are Alpha-Fetoprotein (AFP), C-Reactive Protein (CRP), vascular endothelial growth factor (VEGF). In this work, we select the vascular endothelial growth factor to be our investigated object. In general, the regular method of VEGF detection is Enzyme-linked immunosorbent assay (ELISA). In this research, we studied the magnetic relaxation of VEGF coated magnetic nanoparticle, which is labeled as Fe3O4-antiVEGF, during the association with the antigen by low fields nuclear magnetic resonance system (LFNMR). We hoped that the sensitivity of this method can be comparable to or even beyond the ELISA, and this technique could be applied to clinical trials.


Keywords: Nuclear Magnetic Resonance, Low Fields, Magnetic Nanoparticles