Mechanical Control of Enzymes Using DNA Molecular Springs
Chiao-Yu Tseng1,2*, Andrew Wang2, Giovanni Zocchi2
1Physics, Academia Sinica, Taipei, Taiwan
2Physics, University of California, Los Angeles, United States of America
* presenting author:曾喬毓,
Enzymes are involved in thousands of biological processes and they are essential to life. In a catalytic cycle, the binding of substrate often drives conformational motion, which is critical for the following chemical reaction. To understand this mechanics, our approach is to use a DNA molecular spring to mechanically stretch an enzyme and measure its enzymatic response. A large part of our work was done on Guanylate Kinase (GK), which showed a decrease in enzymatic activity in response to the mechanical perturbation provided by a DNA spring. Moreover, its enzyme kinetics parameters can be affected separately, depending on where the mechanical stress is applied. Recently, we succeeded in controlling Renilla Luciferase (RLuc) with the same strategy. A DNA spring is demonstrated as a general tool to control the activity of an enzyme, and more generally, to explore the mechanical properties of a protein. In addition, protein-DNA molecules may be developed into interesting biosensors. We have showed proof of concept of RLuc-DNA molecule as a sensitive probe for specific DNA targets.

Keywords: Enzyme, DNA spring, Mechano-chemistry, biosensor