Mass Spectrometry: an emerging tool in biology and chemistry
Wen-Ping Peng1*
1Department of Physics, National Dong Hwa University, Hualien, Taiwan
* presenting author:彭文平,
In this talk, I will present the mass spectrometry techniques applied in biology and chemistry. Since the advent of first mass spectrometer by J.J. Thomson and F.W. Aston, isotopes can be measured and applied to earth science and chemistry. German physicist, Wolfgang Paul, invented quadrupole mass spectrometer and quadrupole ion trap in the 1950s. The quadrupole physics are clearly understood in theory; however, the exact transmission of molecular ions is still not comprehended. Here I present our new findings of measuring the ion velocity distribution of proteins, peptides, and fullerene molecules from matrix assisted laser desorption/ionization (MALDI) and laser desorption/ionization (LDI) ion sources with and without electric quadrupole fields. We examined the correlation of ion velocity (i.e. kinetic energy), RF voltage and frequency, bath pressure, stability parameter (qz) and pseudo potential well and found it affects ion transmission.
In application, mass-selected ions are deposited on the well-defined surface and their catalytic reactions are demonstrated in monolayer thickness [1]. With nanodiamond and MALDI time-of-flight mass spectrometry technique, we found an early secreted cell filtrate protein 10 (CFP-10) could be treated as a candidate of mycobacterium tuberculosis complex (MTBC) biomarkers [2]. The performance of CFP-10 biomarker was evaluated in a medium amount of 312 MTBC and 52 nontuberculous mycobacteria (NTM) samples. The sensitivity and specificity are 97.4% and 100%, respectively, which is the highest as compared to those of conventional biomedical approaches. This revealed that the nanodiamond-extracted CFP-10 protein may represent a promising MTBC biomarker. Moreover, quantitative measurement of the cellular uptake of metal nanoparticles and nonmetal nano-/microparticles during cell endocytosis is demonstrated with quadrupole ion trap mass spectrometer [3]. Finally, electrospray ionization coupled to native mass spectrometry (MS) has evolved into an important tool in structural biology to decipher the composition of protein complexes. We present how to correctly assign mass and charge of very complicated mass spectra of hepatitis B virus with a game-theory-based search engine [4, 5].

[1] Peng et al., Phys. Chem. Chem. Phys., 2011, 13, 267–275 (2011)
[2] Peng et al., Anal. Chem. 84, 7972−7978 (2012)
[3] Peng et al., Angew. Chem. Int. Ed. 49, 3460 –3464 (2010)
[4] Peng et al., Anal. Chem. 85, 11275−11283 (2013)
[5] Peng et al., Anal. Chem. 83, 1960–1968 (2011)

Keywords: mass spectrometry, chemistry, biology, quadrupole, biomarker