Detection of bio-molecular interactions using silicon nanowire field-effect-transistors
Chia-Jung Chu1,2*, Ying-Pin Wu1,3, Pei-Hua Chen1, Ding Huang1, Li-Chu Tsai2, Chii-Dong Chen1
1Institute of Physics, Academia Sinica, Taipei, Taiwan
2nstitute of Organic and Polymeric Materials, National Taipei University of Technology, Taipei, Taiwan
3Graduate Institute of Electronics Engineering and Department of Electrical Engineering, National Taiwan University, Taipei, Taiwan
* presenting author:褚家容,
Silicon nanowire field-effect-transistors (SiNW-FETs) were utilized to detect molecular interactions between bio-molecules. We first demonstrated that application of field alignment on the probing molecules could improve the reliability and enhance the detection sensitivity of nanowire sensors. The sensitivity we achieved for detection of DNA hybridization is 0.1fM 15-bases single strand DNA (ssDNA). In addition, we developed a DNA recovery system by introducing a complementary ssDNA that could remove the target ssDNA from the target-probed DNA. These applications could be further used for real-time detection of a wide variety of bio-molecule sensors.

Keywords: Silicon nanowire field-effect-transistor, Structure ordering, Recoverable probe DNA, Biosensor